• منطقة بودونغ الجديدة ، شنغهاي ، الصين .
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Cryo- EM structure of the mycobacterial 70S ribosome in …

Ribosome hibernation is a key survival strategy bacteria adapt under stress. Here, cryo- EM structure of mycobacterial 70S ribosome with hypoxia stress-induced factor RafH suggests the molecular ...

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Computer-controlled linear pH gradient for high …

Computer-controlled linear pH gradient for high-performance liquid chromatographic fractionations of aromatic carboxylic acids and of humic and fulvic acids ... Then, this gradient was applied to fractionations of a series of aromatic carboxylic acids as well as to a differentiation of four humic acids and one fulvic acid. Recommended …

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Co-fractionation–mass spectrometry to characterize native

Mitochondrial protein assemblies are vital for neuronal and brain function. This protocol describes a co-fractionation–mass spectrometry platform to study native motochondrial assemblies in ...

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Membrane Fractionation by Isopycnic Sucrose Density Gradient …

Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia coli. Authors: Elisabete C ... then the membrane fractions are collected by ultracentrifugation and separated at high speed by exploiting the differences in membrane density. The fractions obtained are analyzed for LPS ...

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Fractionation of Cells

Fractionation of Cells. Although biochemical analysis requires disruption of the anatomy of the cell, gentle fractionation techniques have been devised to separate the various cell components while preserving their individual functions.

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Isotopic fractionation of the major elements of molten …

At early times when the magnesium flux is overwhelmingly due to the steep composition gradients, the isotopic fractionations are negative. However, as the diffusive core develops and the chemical gradient declines, the thermally driven flux of magnesium becomes proportionately more important.

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Changes in soil organic carbon contents and fractionations of forests

Request PDF | Changes in soil organic carbon contents and fractionations of forests along a climatic gradient in China | Background: Soil organic carbon (SOC) is a large reservoir of terrestrial ...

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Efficient micro-scale basic reverse phase peptide …

Here we describe a micro-scale basic reverse phase liquid chromatographic (bRPLC) fractionation method that offers high reproducibility and efficiency for peptide …

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Isotopic fractionation in non‐equilibrium diffusive …

Firstly, theoretical transport fractionations likely govern transport rates, but may not be observed due to confounding effects of concentration gradients. Secondly, …

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Thermodiffusion driven element and isotope fractionations: …

Request PDF | Thermodiffusion driven element and isotope fractionations: A remarkable differentiation mechanism in silicate systems | Whether thermodiffusion, a kind of diffusion driven by thermal ...

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Fabrication, tuning, and high-gradient testing of an X-band …

X-band high-gradient linear accelerators are a challenging and attractive technology for compact electron linear-accelerator facilities. The Very Compact Inverse Compton Scattering Gamma-ray Source (VIGAS) program at Tsinghua University will utilize X-band high-gradient accelerating structures to boost the electron beam from 50 to 350 …

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Persistent organic pollutants in global surface soils: …

Global fractionations include latitudinal and longitudinal fractionations. Latitudinal transect is the major reason for POPs to be fractionated into the polar surface media. Decreasing temperature gradient along the latitude prompts accumulation of POPs in cold polar regions (polar cold trapping).

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9 Trouble Shooting and Large-Scale Techniques in …

comes from a temperature gradient which is too steep (i.e., large values of L1 T per em column length). Solvent/nonsolvent gradients which are too steep hinder the full adjustment of the sol-gel equilibrium, especially in the high-MW range. Flow rates too high or low influence the stepwise column extraction less than the other procedures.

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Practical considerations for prostate hypofractionation in the …

Conventionally fractionated external beam radiotherapy is an effective treatment for localized prostate cancer, but long treatment programmes result in a substantial burden to patients and the ...

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High-Performance Liquid Chromatography | SpringerLink

High-performance liquid chromatography (HPLC) was developed during the 1960s as a direct offshoot of classic column liquid chromatography through improvements in the technology of columns and instrumental components (pumps, injection valves, and detectors). Originally, HPLC was the acronym for high-pressure liquid …

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Efficient micro-scale basic reverse phase peptide …

These results collectively demonstrate that micro-bRPLC affords high reproducibility in proteome inventories and detection of differential protein abundance for label-free quantitation from both 20 μg and 5 μg fractionations.

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8.2: Fractionation and Chromatography Techniques

Fractionation of samples, as the name suggests, is a process of separating out the components or fractions of the lysate. Fractionation typically begins with centrifugation of the lysate. Using low-…

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The Soret effect and isotopic fractionation in high

Here we show how transition-state theory can be used to understand the chemical and isotopic fractionation of elements in high-temperature silicate melts subject …

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Modeling of Elemental Isotopic Fractionations Induced By …

Recent experiments by Richter et al. (1,2) found that significant isotopic fractionations of Mg and Ca (and other elements) are produced in basalt melts that are placed in high temperature thermal ...

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Full article: Peptide fractionation in proteomics approaches

These approaches were compared with peptide–peptide separation, using in the first dimension, either high-pH RP, SCX with a salt gradient, or SCX LC with a pH gradient Citation [21]. Results showed that protein fractionation using RP-high-performance LC (HPLC) and peptide fractionation using high-pH RP-HPLC yielded the …

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Review Fractionation of polysaccharides by gradient non-solvent

The principle of fractionation by gradient non-solvent precipitation. Fractionation by gradient non-solvent precipitation is based on phase separation of polysaccharide solutions. Polysaccharides of different molecular weights or chemical structures have different solubility.

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Variations in hydrogen isotopic fractionation in higher plants …

It is observed that leaf wax δDn-alkane values between major high plant lineages (eudicots versus monocotyledonous species) differed significantly under the same environmental conditions, and biological factors inherited from plant taxonomies have a significant effect on controlling leaf wax Greek letter values in higher plants.

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Parameterization of high magnetic field gradient …

A detailed quantitative analysis of a batchwise magnetic fractionation process for malaria infected erythrocytes using high gradient magnetic fractionation columns …

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(PDF) Changes in soil organic carbon contents and fractionations of

Changes in soil organic carbon contents and fractionations of forests along a climatic gradient in China. January 2019; Forest Ecosystems 6(1) ... and high values of A/O-A represent highly decomposed.

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Changes in soil organic carbon contents and fractionations …

Changes in soil organic carbon contents and fractionations of forests along a climatic gradient in China. Formally Refereed. Authors: Xiaolu Sun, Zuoxin Tang, Michael G. Ryan, Yeming You, Osbert Jianxin Sun: Year: ... Changes in soil organic carbon contents and fractionations of forests along a climatic gradient in China. Forest Ecosystems. 6(1 ...

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8.2: Fractionation and Chromatography Techniques

Fractionation of samples, as the name suggests, is a process of separating out the components or fractions of the lysate. Fractionation typically begins with …

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Fagonia cretica: Identification of compounds in bioactive gradient high …

Plants are alternative source of natural medicines due to secondary active metabolites. Fagonia cretica extracts and Gradient High-Pressure Liquid Chromatography fractionations were checked against multidrug-resistant gastrointestinal pathogens including, Salmonella typhi, Escherichia coli and Shige …

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Computer-controlled linear pH gradient for high …

Semantic Scholar extracted view of "Computer-controlled linear pH gradient for high-performance liquid chromatographic fractionations of aromatic carboxylic acids and of humic and fulvic acids" by K. Ravichandran et al.

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Responses of soil aggregate stability to carbon and nitrogen …

Purpose Soil aggregates regulate soil water and temperature, soil fertilizer, and leaf gas exchange. In desert steppes, precipitation restricts the growth and development of plants, and it affects the availability of soil carbon and nitrogen, thereby influencing soil aggregate stability. However, studies on precipitation influence on the …

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Membrane Fractionation by Isopycnic Sucrose Density Gradient …

Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia coli Methods Mol Biol. ... then the membrane fractions are collected by ultracentrifugation and separated at high speed by exploiting the differences in membrane density. The fractions obtained are analyzed for LPS total ...

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